American Journal of Bioscience and Bioengineering 2015; 3(6): 169-177 170
specialized environment due to complex nutritional
requirements. Additionally, viruses that are known as
obligate intracellular parasites require tissue culture medium
consisting of living cells . Peptone (bacteriological) is
obtained through enzymatic digestion of selected fresh meat.
Due to its high nutrition value it assists the growth of the
various microorganisms and used for the identification of the
bacteria by performing various biochemical tests.
Additionally, due to its nutritious nature, mainly at lower
dilutions, for the recombinant cell lines it has been used as an
additive medium for the production of recombinant
therapeutic protein in high-density perfusion culture of
mammalian cells . The stability of proteins at higher
temperature is a major issue that hampers its applications in
many targeted areas [6, 7]. Bischof et al. reported that it is
imperative to understand that how protein loses stability and
to what extent one can control this through the thermal
environment as well as through chemical and mechanical
modification of the protein structure .
On the other hand, Malmgren modified terrestrial orchid
(MMTO) has been used as tissue culture medium for in vitro
culture of orchids. It is mainly composed of glycine, casein
hydrolysate, and agar as nutrients to support the culture
growth. However, it is known to be hygroscopic in nature
that might affect its end uses as plant tissue culture growth
medium . Therefore, some alternative strategies should be
considered to alleviate the thermal stability of peptone and
hygroscopic nature of MMTO. Recently, biofield energy
treatment was used to modify the physicochemical properties
of various materials.
Biofield energy treatments are comprised of practices
based on subtle energy field and generally it reflects the
concept that human beings are infused with this form of
energy . It was shown that a unique bioenergetic field
surrounds and permeates the human body . This
bioenergetic field controls the human wellbeing and during
disease condition this unique field is depleted . Recently
some medical technologies were deployed to measure this
human biofield . Moreover, biofield energy therapies are
categorized under complementary and alternative medicine
(CAM). CAM is approved by National Centre for
Complementary and Alternative Medicine (NCCAM)/
National Institute of Health (NIH) as an alternative treatment
in health care sector . Therefore, it is envisaged that
human beings have the ability to harness the energy from the
environment/Universe and it can transmit into any object
(living or non-living) around the Globe. The object(s) are
always received the energy and responding in a useful
manner that is called biofield energy.
Mr. Mahendra Kumar Trivedi is known to transform the
characteristics of various living and non-living things using
his unique biofield energy. This biofield energy treatment is
also known as The Trivedi Effect
. This unique biofield
treatment has altered the characteristics of pathogenic
microbes  and improved the production in agriculture
. Moreover, biofield energy has modified the
physicochemical properties of metal , drugs  and
organic products .
After conceiving above-mentioned excellent outcome from
biofield energy treatment and properties of peptone and
MMTO, this work was planned to investigate the impact of
this treatment on its physicochemical properties.
2. Materials and Methods
Peptone and Malmgren modified terrestrial orchid
(MMTO) medium were procured from Himedia Laboratories,
India, and the samples were separated into two parts. The one
part was kept aside as a control sample, while the other part
was subjected to Mr. Trivedi’s unique biofield energy
treatment and coded as the treated sample. Both the groups
were in sealed pack, while the treated group was handed over
to Mr. Trivedi for biofield energy treatment under standard
laboratory conditions. Mr. Trivedi gave the energy treatment
through his energy transmission process to the treated
samples without touching the samples. The control and
treated samples were characterized by different analytical
techniques such as X-ray diffraction, differential scanning
calorimetry, thermogravimetric analysis, Fourier transform
infrared spectroscopy, particle size analyzer, and surface area
analyzer. All the experimental techniques were not assessed
by quantitative analysis with this analytical data, does not
require replication of points or statistical analysis.
2.1. X-ray Diffraction (XRD)
XRD analysis of control and treated peptone was evaluated
using X-ray diffractometer system, Phillips, Holland PW
1710 which consist of a copper anode with nickel filter. XRD
system had a radiation of wavelength 1.54056 Å.
2.2. Differential Scanning Calorimetry (DSC)
The control and treated samples (peptone and MMTO)
were analyzed using Pyris-6 Perkin Elmer DSC at a 10°C
/min heating rate and the air was purged at a flow rate of 5
mL/min. The predetermined amount of sample was kept in an
aluminum pan and closed with a lid. A reference sample was
prepared using a blank aluminum pan. The percentage
change in latent heat of fusion was calculated using
% Change in latent heat of fusion
[∆ ∆ ]
∆ × !""
are the latent heat of fusion
of control and treated samples, respectively.
2.3. Thermogravimetric Analysis-Differential Thermal
A Mettler Toledo simultaneous TGA and differential
thermal analyzer (DTA) was used to investigate the thermal
stability of control and treated samples (peptone and MMTO).
The rate of heating was 5°C /min and samples were heated in
the range of room temperature to 400°C under air atmosphere.